Preparation and characterization of new HPLC stationary phase
Автор:Emad Yousif,Hajer Saad Najem and Shahbaz Maki Год: 2012 Издание:LAP Lambert Academic Publishing Страниц: 52 ISBN: 9783659129209 New stationary phase for high performance liquid chromatography had been prepared by the reaction of congo red solution with amberlite anion exchange. The F.T.I.R elemental analyses have configured the attachment of congo red on the resin as has indicated by the physical appearance of the new resin. The resin capacities of newly prepared resin has calculated the resin capacity for the amberlite-congo red resin was found to average 4.8 meq/ gm and for the amberlite resin 3.0 meq./gm. The new stationary phase has been packed into stainless steel column. This has been done by the special teqnique. The chromatographic performance of the packed column was characterized the number of plate numbers, height equivalent of a theoretical plates, capacity factors, selectivity factor and peak asymmetry were measured by analyzing different analysis on the new column using different mobile phases composition and flow rates. The parameters were measured from analasisi of some amino acids such as...
