Molecular and Biochemical Fingerprinting of Clusterbean
Автор:Sampat R. Kalaskar,Suresh Acharya and S. B. S. Tikka Год: 2014 Издание:LAP Lambert Academic Publishing Страниц: 132 ISBN: 9783659593703 Molecular and biochemical fingerprinting used to identification of superior genotypes are the basic objectives of any crop improvement program. The recently developed molecular markers and isoenzymes assay has opened up new visits for the assessment of fingerprinting among genotypes at molecular and biochemical level. In the present investigation, one multi loci molecular markers viz. RAPD (19 Primers), one single locus molecular markers viz. SSR (19 Primers) and three isoenzyme assay viz. PO, PPO and PAL were used to assess the fingerprinting among 12 genotypes of clusterbean, which includes six resistance and six susceptible to bacterial leaf blight genotypes. Fingerprinting analysis was done with the help of statistical analysis such as marker index, polymorphism information content, dendogram and pi-chart. The present study clearly elucidated the RAPD, SSR and isoenzyme assay could be used as a good tool for detecting fingerprinting and can be precisely used for grouping and...
